Columbus:

The W.I. Mitchell Home Site is the former home of Union Academy's first black principal, W.I. Mitchell, in 1877. The Union Academy was one of the very first, if not the first, school to educate freed slaves when it began in 1865.

The Mississippi University for Women was the nation's first public college for women when it opened in 1884. The university has been operating continuously and now enrolls male students, as well.

The Columbus War Museum is dedicated to soldiers of American wars, both fallen veterans and those who have survived to tell the tales.

Tennessee Williams Home is the birthplace of one of the country's most significant playwrights, Tennessee Williams, in 1911. Williams made history with plays such as The Glass Menagerie and Cat on a Hot Tin Roof. This home also currently serves as the city of Columbus' welcome center.

The Stephen D. Lee Home and Museum was the home of General Stephen D. Lee, crucial Civil War leader and distant relative of the legendary Robert E. Lee. Stephen played important parts in developing the United Confederate Veterans and Sons of Confederate Veterans organizations, as well as in the founding of Mississippi State University. His home is now available for touring and events.

West Point:

The Howlin' Wolf Blues Museum promotes the education of blues music and important Mississippi musicians, especially Chester Arthur "The Wolf" Burnett, a Clay County native.

The Waverly Plantation Mansion is a shining example of Greek revival architecture in a southern antebellum home. Once a self-sustaining community, this mansion had its own lumber mill, gardens and orchards, and a unique unsupported curved staircase. It is one of the south's most photographed homes of its era.

The Wilhite Transportation Museum features everything transportation related in West Point's history, from Native Americans hundreds of years ago to the railroad industry and early cars, including a 1927 Model T Ford car.

Starkville:

The Oktibbeha Heritage Museum is a local history museum featuring a wide variety of exhibits that reflect Starkville over the years. From Civil War memorabilia, local businesses, photographs, antique clothing, and sports displays, the museum has it all.

The Templeton Music Museum at Mississippi State University (MSU) allows visitors to experience the music atmosphere of the 19th-20th centuries in America through Charles H. Templeton's personal collection of music memorabilia.

The Dunn-Seiler Geology Museum at MSU is a great place for visitors who want to learn about various geological specimens and see them up close; on display are Mississippian fossils and replica skeletons, how plate tectonics work, many different types of rocks, and much more.

The Wade Clock Museum at MSU showcases the personal collection of Charles and Gladys Wade, which amounts to more than 400 American and European clocks, some which date back to the 1700s. The Wade's clock assortment is so large, in fact, that almost every American manufacturer is displayed.

The Mississippi Entomological Museum, part of the MSU Department of Entomology and Plant Pathology, contains over 1 million insect specimens from all over the world, including far-reaching locales such as Fiji and the Seychelles. Also at the museum is the Ross E. Hutchins insect and nature photography collection and a group of folk who enjoy photographing moth species for education and research, known appropriately as the Moth Photographers Group.